300

Polymerase Chain Reaction Quiz

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1. Which of the following is an advantage of single-primer PCR?

   Less synthetic DNA is incorporated into the product.

   Unknown sequence adjacent to a known sequence can be amplified with a single primer.

   No primer-dimer artifacts.

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2. Which end of a PCR primer can be extended by polymerase?

   The 1' end.

   The 2' end.

   The 3' end.

   The 4' end.

   The 5' end.

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3. Which nuclease activity does Taq polymerase NOT have?

   5' to 3'

   3' to 5'

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4. Which of the following significantly inhibits PCR?

   12% sucrose

   Wooden toothpicks made of birch.

   0.2mM cresol red.

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5. What is the minimum time required for denaturation in rapid-cycle PCR?

   One minute.

   30 seconds.

   Less than one second.

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